Working Group 2

Artificial models – understanding bioadhesion in vitro

Focus

  • To design and characterise artificial micro- and nanostructures supporting adhesion
  • To characterize and model adhesion properties of biomolecules
  • To determine and characterise adhesion properties in hybrid systems

Tasks

Task 2.1: Topography design/evaluation
Test the role of hierarchical structures (macro- micro– nano level) defined in WG1 in combination with biomolecules and test their contribution to adhesion at the interface. Artificial micro- and nanostructures of similar geometry will be designed and built using lithography techniques, 3D printing, electrospinning, Focus Ion Beam scanning electron microscopes, etc., and test their role in adhesion using a broad range of force measurement techniques.

Task 2.2: Biomolecule characteristics/processing
The goal of this task is to understand, measure and model the ‘stickiness’ of bioadhesive molecules in detail. Advanced molecular simulations and detailed measurements i.e. by surface plasmon resonance, atomic force microscopy and others will be employed (according to WG 1) to the artificially developed
biomolecules to characterise substrates and the key proteins, generate in-depth information relating to adsorption/adhesion to different chemical functionalities and the nanomechanical properties of the molecule. As tests with purified biomolecules may not be sufficient in terms of amount or polymer length, production of biomolecules via chemical synthesis or recombinant techniques will be discussed with experts from other relevant disciplines and implemented into this task.

Task 2.3: Hybrid systems
To understand the mutual influence and effect of different biomolecules and bonding principles, models from task 2.1 and 2.2 will be combined into hybrid and multi-response systems such as hydrogels, polymers or other materials. Characterization and measurement of these systems will serve to provide additional knowledge on basic bonding principles.

Working Group Coordinators

Leader: Seda Kizilel (Koç University, Turkey) skizilel@ku.edu.tr

Vice-Leader: Päivi Laaksonen (Aalto Universit, Finland) paivi.laaksonen@aalto.fi

Task 2.1 Leader

Marleen Kamperman
Wageningen University
Netherlands
marleen.kamperman@wur.nl

Task 2.2 Leader

Zhenyu Jason Zhang
University of Birmingham
United Kingdom
z.j.zhang@bham.ac.uk

Task 2.3 Leader

Arita Dubnika
Riga Technical University
Latvia
arita.dubnika@rtu.lv