Task 1.2: Composition/Profiling
The focus is (i) to identify and characterize structures of dry and wet adhesives chemically, (ii) to develop sample preparation and extraction procedures for wet adhesives, (iii) to discuss and adapt general chemical characterization approaches of the different glues including their biomolecular composition, amino acid/monosaccharide sequence and spectral features and (iv) to optimise the identification of the most abundant (glyco-) proteins and peptides. State-of-the-art technologies will include chromatography (i.e. High-performance liquid chromatography), biochemical techniques (i.e. SDS-Page Gel electrophoreses, Matrix-assisted laser desorption/ionization time-of-flight mass spectrometer/imaging) as well as molecular tools (i.e. de novo transcriptome/genome sequencing), which are available in the different labs of the ENBA participants.